With a focus on the application of molecular concepts to diagnostic purposes, the text explains and illustrates the use and interpretation of molecular-based assays in patient care.

Table of Contents

• Section I. Fundamentals of Molecular Biology: An Overview
• 1. Nucleic Acids and Proteins
• 2. Gene Expression and Epigenetics
• Section II. Common Techniques in Molecular Biology
• 3. Nucleic Acid Extraction Methods
• 4. Resolution and Detection of Nucleic Acids
• 5. Analysis and Characterization of Nucleic Acids and Proteins
• 6. Nucleic Acid Amplification
• 7. Chromosomal Structure and Chromosomal Mutations
• 8. Gene Mutations
• 9. DNA Sequencing
• Section III. Techniques in the Clinical Laboratory
• 10. DNA Polymorphisms and Human Identification
• 11. Detection and Identification of Microorganisms
• 12. Molecular Detection of Inherited Diseases
• 13. Molecular Oncology
• 14. DNA-Based Tissue Typing
• 15. Quality Assurance and Quality Control in the Molecular Laboratory
• Appendix A. Study Question Answers
• Appendix B. Answers to Case Studies
• Glossary